Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
Alkali Scientific Radiant™ Probe 1-Step No-ROX qPCR Kit, 300 Reactions, (3 X 1mL 2X Mix, 3 X 0.2mL 20X RTase)
Alkali Scientific offers Radiant™ Probe 1-Step No-ROX qPCR Kits, a high-performance portfolio of 2X ready-to-use real-time PCR reagents designed for best-in- class quantitative PCR. Radiant™ qPCR Kits are engineered for robust real-time PCR with earlier quantification cycle values (Ct), industry-leading sensitivity (increased limit of detection) and exceptional speed (rapid extension rates). The proprietary buffer system allows for highly efficient amplification of GC-rich and AT-rich sequences in addition to dramatic improvements in PCR sensitivity in low- copy assays and PCR conditions conducive to ultra-fast amplification. The Radiant™ Probe 1-Step No-ROX qPCR Kit utilizes a proprietary, Green non-inhibitory intercalating dye for robust, fluorescent detection.
Sigma Aldrich Fine Chemicals Biosciences KiCqStartR Probe qPCR Read
KiCqStart Probe qPCR ReadyMix isanadvanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust SOLUTIONon that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries including TaqMan(R) hydrolysis probes. KiCqStart Probe qPCR ReadyMix is provided as a 2X concentrated ready-to-use reaction cocktail that contains all required reaction components except primers probe(s) and DNA template. The light blue color of an inert tracer dye simplifies reaction assembly in white or clear plates and helps to minimize pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.A key component of KiCqStart Probe qPCR ReadyMix is an ultra-pure processive thermostable DNA polymerase that is free of detectable E. coli DNAA§. KiCqStart Probe qPCR ReadyMix is ideal for demanding qPCR applications such as bacterial pathogen detection w
Sigma Aldrich Fine Chemicals Biosciences Protector RNase Inhibitor
Protector RNase Inhibitor inactivates a wide spectrum of RNases includingRNase ARNase BRNase T2Thus Protector RNase Inhibitor can help prevent RNase degradation in any application where RNases could cause problems. for instance it canprotect mRNA during cDNA synthesis reactions RT-PCR (in conventional thermal cyclers and qPCR systems) or in vitro transcription/translation reactionsprotect viral RNA during in vitro virus replicationinhibit RNases during RNA isolation and purificationbe used in RNase protection assayshelp prepare RNase-free antibodiesNote Protector RNase Inhibitor does not interfere with enzymes commonly used to prepare or analyze RNA.
STEMCELL Technologies Human Mesenchymal Stem Cell qPCR Array, Size: 1 Unit
For characterization of human MSCs and their differentiation to chondrogenic, adipogenic, or osteogenic lineages
Alkali Scientific Radiant™ Green Hi-ROX qPCR Kit, 10,000 Reactions, (100 x 1mL)
Radiant™ Green Hi-ROX qPCR Kit, 10,000 Reactions, (100 x 1mL)
Takara Bio Advantage® RT-for-PCR Kit, 100 Rxns
Complete kit for the synthesis of first-strand cDNA from total RNA or polyA+ RNA. The kit contains sufficient reagents for 100 first-strand cDNA synthesis reactions.
Integrated DNA Technologies Inc PrimeTime Gene Expression Master Mix, High PCR Efficiency >90%, Compatible With Various qPCR Systems, 5 mL
The PrimeTime Gene Expression Master Mix is a 2X concentrated solution that enhances accuracy and efficiency in qPCR. It guarantees PCR efficiencies over 90% and R² values above 0.99, making it reliable for gene expression analysis. The mix features exceptional stability at room temperature and high temperatures, reducing shipping costs and environmental impact. Its broad compatibility with various primers and probes differentiates it in the market, perfect for both routine and high-throughput applications.
New England Biolabs, Inc. OneTaq Hot Start 2X Master Mix with Standard Buffer – 100 reactions (50 µl vol)
OneTaq Hot Start 2X Master Mix with Standard Buffer is an optimized blend of Taq and Deep Vent DNA Polymerases combined with an aptamer-based inhibitor. This enzyme blend is ideally suited to routine PCR applications from a variety of templates, including pure DNA solutions, bacterial colonies and cDNA products. The 3' to 5' exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase. The hot start nature of the enzyme offers convenience with decreased interference from primer dimers and secondary products. The convenient master mix formulation contains dNTPs, MgCl2 and other buffer components and stabilizers listed below, requiring only the addition of primers and DNA template for robust amplification.
Alkali Scientific Radiant™ Probe 1-Step Lo-ROX qPCR Kit, 100 Reactions, (1 X 1mL Probe 1-Step Lo-ROX 2X qPCR Mix, 1 X 0.2mL 20X RTase with RNase Inhibitor)
Alkali Scientific offers Radiant™ Probe 1-Step Lo-ROX qPCR Kits, a high-performance portfolio of 2X ready-to-use real-time PCR reagents designed for best-in- class quantitative PCR. Radiant™ qPCR Kits are engineered for robust real-time PCR with earlier quantification cycle values (Ct), industry-leading sensitivity (increased limit of detection) and exceptional speed (rapid extension rates). The proprietary buffer system allows for highly efficient amplification of GC-rich and AT-rich sequences in addition to dramatic improvements in PCR sensitivity in low- copy assays and PCR conditions conducive to ultra-fast amplification. The Radiant™ Probe 1-Step Lo-ROX qPCR Kit utilizes a proprietary, Green non-inhibitory intercalating dye for robust, fluorescent detection.
Cytek Biosciences Inc CYBRFast™ 1-Step RT-qPCR Hi-ROX Kit
CYBRFast™ 1-Step RT-qPCR Hi-ROX Kit ..... Cat. No. 31-5202-0300R ..... Size 300 rxns
New England Biolabs, Inc. Luna® Universal Probe One-Step RT-qPCR Kit – 1000 reactions
In the Luna Universal One-Step Probe RT-qPCR Kit, Hot Start Taq DNA Polymerase is combined with a novel WarmStart-activated reverse transcriptase, allowing dual control of enzyme activity via reversible, aptamer-based inhibition. This temperature-dependent activation helps to prevent undesirable non-specific priming and extension prior to thermocycling, providing added security for setting up reactions at room temperature. The engineered WarmStart Luna Reverse Transcriptase also possesses higher thermostability than many other RTs, allowing an optimal reaction temperature of 55°C. For difficult targets/templates, higher RT step temperatures of up to 60°C can be used without compromising Luna performance. Note that to ensure full activation of the WarmStart Luna RT, incubation at temperatures lower than 50°C is not recommended.
- Non-interfering, visible tracking dye helps to eliminate pipetting errors
New England Biolabs, Inc. Luna Cell Ready One-Step RT-qPCR Kit – 100 reactions
The Luna Cell Ready One-Step RT-qPCR Kit provides all the necessary components for direct, dye-based RNA detection and quantitation, bypassing the need for RNA extraction and purification. The Luna Cell Ready One-Step RT-qPCR Kit is composed of: 1) the Luna Cell Ready Lysis Module and 2) the Luna Universal One-Step RT-qPCR Kit. Coordinating the actions of DNase I and the Luna Cell Ready Protease, the Luna Cell Ready Lysis Module offers a simple alternative workflow resulting in effective cell lysis, RNA release, and genomic DNA removal simultaneously in a 15-minute protocol. The Lysis Module includes a unique Luna Cell Ready RNA Protection Reagent that maintains RNA integrity during cell lysis. The lysis capacity spans 10 -100,000 cells in a 50 µl lysis reaction. Up to 2 µl of lysate (equivalent to RNA from 0.2 - 4,000 cells) can be transferred into 20 µl downstream RT-qPCR reactions.
New England Biolabs, Inc. Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit - 96 rxn
The Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit is optimized for real-time qualitative detection of SARS-CoV-2 nucleic acid using hydrolysis probes. It features the Luna Probe One-Step RT-qPCR 4X Mix with UDG, an optimized SARS-CoV-2 Primer/Probe mix containing primers and probes specific to two regions of the SARS-CoV-2 virus N-gene, and a positive control template. The probes have been modified to contain different fluorophores (N1, HEX; N2, FAM) to enable simultaneous observation on two different channels of a real-time instrument. To ensure the integrity of the input material and absence of inhibition, an internal control (IC) primer and probe set, designed to amplify the human RNaseP gene, is also provided in the primer mix. The reverse primer of this target has been modified from the CDC design to target an exon/exon boundary to reduce background amplification from possible contaminating genomic DNA. Amplification of the IC is observed in the Cy5 channel.